Figure 3

Instability of the aptamer cassette in transduced CEMx174 cells. Genomic DNA from transduced cells and plasmid DNA from the different vectors were PCR amplified with a forward primer from GFP (3'GFP-for) and a reverse primer from the untranslated region of the vector (3' UTR-rev). PCR reactions were separated by gel electrophoresis and stained with ethidium bromide. The expected size of the MMP-70.15 amplicon is 654 bps; the expected size for the other bands varies depending on the length of the aptamer. The DNA ladders are either the 100 bp DNA ladder or the 1 kb DNA ladder from NEB.