Figure 2

Inhibition of HIV-1 _NL4-3 in aptamer transduced CEMx174 cells. A) Transduction of the CD4⁺ cell line CEMx174. CEMx174 cells were transduced with each MMP-aptamer vector by spinoculation and overnight incubation with Phoenix (GaLV) supernatants. Cells were washed and expanded for 48 hours before analysis by flow cytometry. The dot plots indicate GFP expression versus side scatter characteristics for the empty vector (left plot), 70.15 vector (left center), 70.8 vector (right center), and 80.55 vector (right plot) before sorting. The percentage of GFP⁺ cells and the mean fluorescent intensity are indicated in the upper right corner of the plot. B) Challenge of aptamer-transduced CEMx174 cells with HIV-1_NL4-3. Aptamer-transduced CEMx174 cells (empty, filled circle; 70.15, yellow square; 70.8, green diamond; 80.55, blue triangle) were sorted for expression of GFP, infected with HIV-1_NL4-3 (MOI = 0.01 TCID₅₀ per cell) and followed over 14 days for production of p24 Gag by ELISA.