Figure 3
From: Glutathione and growth inhibition of Mycobacterium tuberculosis in healthy and HIV infected subjects
Growth of H37Rv in whole blood cultures of HIV patients. Blood from HIV positive subjects was diluted at the following proportion, 300 μl of blood was diluted to 1 ml with RPMI. One milliliter of diluted blood was added to each well of 12 well tissue culture plates. Blood cultures were treated with none (Fig 3a), or 10 mM NAC (Fig 3b) or NAC, 500 μM BSO, (Fig 3c) for 24 h. Blood cultures were infected with processed H37Rv. Infected blood cultures were terminated at 2 h & 48 h after infection, to determine the intracellular viability of H37Rv. Intracellular viability of H37Rv was determined by plating the diluted blood cell lysate on 7H11. Data in Figure 3a, b, and c, are averages from four, eight and three subjects, respectively.