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Figure 2 | AIDS Research and Therapy

Figure 2

From: Glutathione and growth inhibition of Mycobacterium tuberculosis in healthy and HIV infected subjects

Figure 2

Spectrophotometric assay of GSH in PBMC (Fig 2a) and RBC (Fig 2b), derived from healthy and HIV positive subjects: GSH assay kit was procured from Calbiochem. PBMC and RBC lysates (from HIV-infected and healthy subjects), were mixed with equal volume of ice cold 5% metaphosphoric acid (MPA) and centrifuged at 3000 rpm for 15 minutes. Supernatants were used for GSH assay, as per manufacturer's instruction. Samples were also used for protein assay by Bradford's method using Bio Rad reagent.

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