Figure 1

Growth of H37Rv in unstimulated (Fig 1a), IFN-γ, LPS (Fig 1a), and NAC treated HMDM (Fig 1b). Human monocytes from peripheral blood were maintained in vitro in RPMI containing 5% AB serum, for 7 days for differentiation to macrophages. HMDM were infected with H37Rv at moi of 10:1 and maintained in media alone (Fig 1a) or in media containing IFN-γ, LPS (100 U/ml and 1 μg/ml), respectively, (Fig 1a) or media containing, NAC 10 mM (Fig 1b). Infected macrophages were terminated at 4 h & 7 d after infection to determine the intracellular growth of H37Rv. Intracellular colony counts of H37Rv were determined by plating lysed cultures on Middlebrook 7H11. Fig 1a, are means from six different experiments performed in triplicate. Fig 1b, are means from three different subjects performed in triplicate. (Fig 1c) Whole blood Infection of H37Rv. Blood from healthy individuals was diluted at the following proportion, 300 μl of blood was diluted to 1 ml with RPMI. One milliliter of diluted blood was added to each well of 12 well tissue culture plates. Blood cultures were treated with none or NAC (10 mM) or NAC, BSO (500 μM) for 24 h. Blood cultures were infected with processed H37Rv. Infected blood cultures were terminated at 2 h & 48 h after infection, to determine the intracellular viability of H37Rv. Intracellular viability of H37Rv was determined by plating the diluted blood cell lysates on 7H11. Data in Figure 1c are averages from seven subjects performed in triplicate.