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Table 2 Operational comparison of the two methodologies

From: Comparison of capillary based microflurometric assay for CD4+ T cell count estimation with dual platform Flow cytometry

Sr. No. Parameter EasyCD4 System Conventional flow cytometry (FACSort)
1. Ease of performance Excellent Good
2. Volume of blood required/test 10 μl 100 μl
3. Reagents required/test   
  a. Antibodies 1 μl/sample 20 μl/sample
  b. Sheath fluid --- 50 ml/sample
     c. RBC Lysing solution 18 μl/sample 120 μl/sample
4. Generation of waste/test 200 μl/sample 18 ml/sample
5. Time required to process one sample 35 minutes 90 minutes
6. Routine maintenance: Cleaning procedure Daily (5 minutes) Monthly (-----) Daily (20 minutes) Monthly (90 minutes)