Figure 5

A: Individual phenotypic responses of HIV-negative and HIV-positive subjects to pp65+IE-1 or Gag+Env stimulation. Grey bars represent the mean (for 20 HIV-negative or 19 HIV-positive subjects) of the absolute number of cells for each of the 32 phenotypes, as listed at the bottom of the figure. Black bars represent the background, as mean of the absolute number of cytokine-positive cells in the unstimulated samples, for each phenotype. In both cases, error bars represent SEM. Phenotypes are grouped horizontally as CD4+ or CD8+, and by their cytokine profile. Within each subgroup, phenotypes are arranged from naïve (left) to terminal effector (right), with intermediates arranged by order of potential for IL-2 production in healthy donors (see Figure 6, top panel). Results are shown for the response of HIV-negative subjects to pp65+IE-1 stimulation (top), the response of HIV-positive subjects to pp65+IE-1 stimulation (middle), and the response of HIV-positive subjects to Gag+Env stimulation (bottom). B: Individual phenotypes of total CD4+ or CD8+ T cells. Each bar represents the mean (for 19 HIV-positive subjects) of the absolute number of cells displaying each of the phenotypes, as listed at the bottom of the figure. Error bars represent SEM. Results are shown for HIV-positive patient PBMC stimulated with Gag+Env peptide mix, for direct comparison to the HIV-specific response shown in A.