Figure 1
From: IL-2 production correlates with effector cell differentiation in HIV-specific CD8+ T cells
Hierarchical gating strategy. After applying an acquisition threshold on CD3, gate A was constructed to include CD3 dim cells. CD3+ low-scatter cells were identified either as CD4+ CD8- or CD4- CD8+ (gates B and C, which also included dim positive cells). Cytokine+ cells in gates B and C were identified either as total IFNγ+ or IFNγ+ IL-2+. These two classes of cytokine-producing cells were determined to be either CD27- or CD27+, and then further separated by expression of CD28 and CD45RA. Gates R through WW represent the 32 ultimate phenotypes compared in this study. The example shown is from a pp65+IE-1 stimulated HIV-negative subject.