Figure 3
From: A nucleolar localizing Rev binding element inhibits HIV replication
U16RBE in vivo activity. Some of the CEM clones expressing the U16RBE RNA decoy along with the parental CEM cells were challenged with HIV-1 NL4-3 at an m.o.i of 0.001.CEM clones expressing either a nucleolar anti-HIV-1 hammerhead ribozyme wt (U16Rz wt 11) or the mutant disabled mutant version (U16Rz mutant 4) were used respectively as positive and negative controls for inhibition of HIV-1 infection [18]. On days 1, 10 and 17 day post-infection the supernatants from these cell cultures were collected and assayed for HIV-1 p24Gag accumulation. The data presented represent an average of two independent challenge experiments, and the standard errors for each point are around 5%