HIV-1 reverse transcriptase mutations that confer decreased in vitro susceptibility to anti-RT DNA aptamer RT1t49 confer cross resistance to other anti-RT aptamers but not to standard RT inhibitors

Table 2 Sensitivity of aptamer-resistant RTs to NRTIs and NNRTIsAssays were performed as described in the text. Data represent mean ± SEM of three independent experiments.

	WT		N255D		N265D		Dbl
Inhibitor	^aIC₅₀, μM	^bRatio	IC₅₀, μM	Ratio	IC₅₀, μM	Ratio	IC₅₀, μM	Ratio
AZTTP	1.83 ± 0.25	1	2.67 ± 0.09	1.45	1.74 ± 0.28	0.9	2.43 ± 0.26	1.3
ddATP	0.93 ± 0.18	1	1.07 ± 0.11	1.2	0.84 ± 0.04	0.9	0.91 ± 0.07	1
ddCTP	0.88 ± 0.20	1	0.69 ± 0.07	0.8	0.72 ± 0.17	0.8	0.96 ± 0.09	1.1
3TCTP	4.37 ± 0.87	1	2.51 ± 1.04	0.6	5.02 ± 1.22	1.1	2.69 ± 0.95	0.6
d4TTP	0.79 ± 0.05	1	0.83 ± 0.14	1	0.64 ± 0.12	0.8	0.91 ± 0.10	1.2
Nevirapine	0.10 ± 0.01	1	0.06 ± 0.02	0.6	0.09 ± 0.03	0.9	0.07 ± 0.01	0.7
Delavirdine	0.37 ± 0.02	1	0.64 ± 0.03	1.7	0.36 ± 0.01	1	0.31 ± 0.01	1

ISSN: 1742-6405