| WT | N255D | N265D | Dbl |
---|
aTRTI | bIC50, nM | cRatio | IC50, nM | Ratio | IC50, nM | Ratio | IC50, nM | Ratio |
RT1t49d RT26f | 1.6 4.0 ± 0.05 | 1 1 | 7.9 7.6 ± 0.1 | 4.9 1.9 | 17.4 11.2 ± 0.1 | 10.9 2.8 | 245 24 ± 0.1 | 153 6 |
RT4f | 38 ± 1.2 | 1 | 80 ± 3.7 | 2.1 | 1015 ± 16 | 27 | > 1000 | > 27e |
RT6f | 19.6 ± 0.1 | 1 | 26 ± 0.7 | 1.3 | 87 ± 2.2 | 4.4 | 142 ± 3.4 | 7.2 |
RT8f | 19.5 ± 0.3 | 1 | 2.0 ± 0.02 | 0.1 | 17.2 ± 0.2 | 0.9 | 3.0 ± 0.02 | 0.1 |
RT10f | 82 ± 2.5 | 1 | 57 ± 1.4 | 0.7 | 923 ± 8.9 | 11 | 509 ± 4.2 | 6 |
Rknot 1.1f | 1.4 ± 0.02 | 1 | 0.8 ± 0.01 | 0.8 | 2.5 ± 0.04 | 2 | 4.5 ± 0.08 | 4 |
- aDescribed in references 23 and 25.
- bConcentration of aptamer at which 50% of the activity was inhibited.
- cFold increase or decrease over the IC50 for the wild type (WT) RT.
- dReproduced from Fisher et al. [25] eAt the highest concentration of aptamer tested (1000 nM), the Dbl mutant retained 70% of its activity, thus the actual IC50 would be much higher.
- fAptamer sequences: RT1t49: 5' ATCCGCCTGATTAGCGATACTCAGAAGGATAAACTGTCCAGAACTTGGA3'
- RT26: 5'ATCCGCCTGATTAGCGATACTTACGTGAGCGTGCTGTCCCCTAAAGGTGATACGTCACTTGAGCAAAATC ACCTGCAGGGG3'
- RT4:5'ATCCGCCTGATTAGCGATACTTTAGCAAAGTTGAAGCCGGACTAACAAGCTCTACGACTTGAGCAAAATCA CCTGCAGGGG3'
- RT6: 5'ATCCGCCTGATTAGCGATACTCAGGCGTTAGGGAAGGGCGTCGAAAGCAGGGTGGGACTTGAGCAAAATCA CCTGAGGGG3'
- RT8:5'ATCCGCCTGATTAGCGATACTAGCCAGTCAAGTTAATGGGTGCCATGCAGAAGCAACTTGAGCAAAATCA CCTGCAGGGG3'
- RT10:5'ATCCGCCTGATTAGCGATACTTATTTGCCCCTGCAGGCCGCAGGAGTGCAGCAGTACTTGAGCAAAATCA CCTGCAGGGG3'
- Rknot 1.1: 5'GGGAGAUUCCGUUUUCAGUCGGGAAAAACUGAA3'