Figure 6From: HIV-1 resistance conferred by siRNA cosuppression of CXCR4 and CCR5 coreceptors by a bispecific lentiviral vectorLack of interferon induction in siRNA transduced cells. To detect interferon induction in siRNA vector transduced cells, western blot analysis was performed to detect elevated levels of phophorylated PKR. Poly I:C was used to induce interferon as a positive control. Transduced cell extracts were run on 10% SDS-PAGE gels, transferred, and probed with an anti-phospho-PKR antibody. Positive control poly I:C transfected (lanes 1 and 2), non-transduced (lane 3), GFP-alone vector (lane 4), and XHR transduced (lane 5) Magi-X4 cells and XHR transduced Ghost-R5 cells (lane 6). An anti-actin antibody was used as an internal control.Back to article page