Figure 5

Northern analysis to detect siRNA expression in transduced cells. Small RNAs (<200 nt) were extracted from transduced cells and probed with specific primers to detect the expression of siRNAs as described in materials and methods. A) Northern blot to detect the presence of miRNA-16 (~22 bp) as an internal control in GFP-alone vector transduced (lane 2) and XHR transduced Magi-X4 (lane 3) and Ghost-R5 (lane 4) cells. B) siRNA (~21 bp) detection in GFP-alone vector transduced (lane 2) and XHR transduced Magi-X4 (lane 3) and Ghost-R5 (lane 4) cells. Decade markers (lanes A1 and B1).