CD14+monocytes are the main site of HO-1 induction after ex vivo CoPP stimulation. Thawed PBMC samples from Early ART Patients (Table 3) (n = 24) were analyzed for cell surface expression of monocyte markers. (A) Scatter plots showing the expression of CD14 and CD16 after monocyte cell culture in PBS (left) and stimulation with CoPP (right) on UpCell™ plates are depicted (B) Histograms representing the fluorescence intensities of HO-1, CD14, CD16, and CD11c in CD14+ monocytes and in CD11c+ mDCs after treatment with saline or CoPP are shown. Thawed PBMCs were cultured with saline or 25 μM CoPP for 48 hours on UpCell™ plates, harvested, and then stained with antibodies recognizing cell-surface and intracellular myeloid markers. Data from a representative subject are shown. (C) CoPP-induced changes in HO-1 among CD14+ monocytes or CD11c+ DCs. The ****symbol represents a p < 0.0001 for differences in culture conditions (saline vs. CoPP).