Differential glycosylation of envelope gp120 is associated with differential recognition of HIV-1 by virus-specific antibodies and cell infection

Table 1 Epitope specification of tested monoclonal antibodies

Monoclonal antibody	Epitope structure	Epitope	Direct involvement of glycans in epitope formation	Different reactivity with individual gp120 glycoforms*	Glycan-shielding effect**	Dilution corresponding to linear range in ELISA (μg per ml)*	References
268 D4	V3 loop tip HIGPGR	linear	-	+	+	0.03	[51, 70]
257 D4	V3 loop stem KRIHI	linear	-	+	+	0.01	[51, 70]
F425 B4e8	V3 loop tip involving GPGRA, Ile³⁰⁹, and Phe³¹⁷	linear	-	-	+	0.04	[52, 71]
447-52D	V3 loop tip GPGR plus amino acids at N-terminal segment of the V3	conformational	-	-	+	0.07	[72, 73]
19b	V3-loop tip -I----G--FY-T	linear	-	+	+	0.01	[74]
2G12	Man α1,2 Man-containing oligo-mannose N- glycans on C2, C3, V4, and C4	conformational	+	+	+	0.04	[75, 76]
PGT 121	V3 base together with multiple surrounding glycans	conformational	+	+	-	0.15	[77]
PG16	Epitope dependent on several glycosylation sites in the V1, V2, V3 loop mostly high-mannose	conformational	+	+	-	7	[45, 78]
PG9	Epitope dependent on several glycosylation sites in the V1, V2, V3 loop mostly high-mannose	conformational	+	+	-	6	[45, 78]
b12	CD4 binding site	conformational	-	+	-	0.01	[79, 80]
VRC03	CD4 binding site	conformational	-	+	-	7	[81, 82]
VRC01	CD4 binding site	conformational	-	+	+	0.15	[81, 82]

*This study, conclusions based on the differences in mAb binding to individual gp120 glycoforms; ** this study, conclusion based on the partial N-glycan removal effect on mAb binding.

ISSN: 1742-6405