Figure 2

Comparison of reactivities of selected HIV-1 gp120-specific monoclonal antibodies with native and deglycosylated recombinant HIV-1 gp120 analyzed by ELISA. ELISA plates were coated with anti-penta-His antibody followed by capture of equal amounts of gp120 produced in HEK 293T (abbreviated as 293), Jurkat, RD, HepG2, or CHO cells (0.05 μg gp120 per well). gp120 preparations were in native form (black columns) or deglycosylated with PNGase F (grey columns). Monoclonal antibodies 268 D4, F425 B4e8, 257 D4, 447-52D, 19b, 2G12, b12, PGT121, PG16, PG9, VRC03, and VRC01 were added and the bound IgG antibodies were detected with HRP-conjugated goat anti-human IgG. Optimal dilutions for each antibody were determined in preliminary experiments using gp120 produced in HEK 293T cells and it is specified in the Table 1. Values correspond to mean absorbance ± SD. Averaged results from three experiments with independently produced recombinant gp120 glycoforms are shown.