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Figure 1 | AIDS Research and Therapy

Figure 1

From: Differential glycosylation of envelope gp120 is associated with differential recognition of HIV-1 by virus-specific antibodies and cell infection

Figure 1

Reactivities of Env-specific monoclonal antibodies with gp120 produced in different cell lines determined by Western blot. (A) Antibody binding to gp120 glycoforms produced in HEK 293T, Jurkat, HepG2, and CHO cell lines was analyzed by Western blot after SDS-PAGE separation under reducing conditions. Western blots were developed with monoclonal antibodies 268 D4, F425 B4e8, 257 D4, 447-52D, 19b, 2G12, and b12. Anti-V5-tag antibody was used to assess the amount of loaded gp120. (B) Densities of individual gp120 glycoform bands obtained from two Western blots after developing with individual monoclonal antibodies were analyzed by ImageJ 1.41a software. Densitometric values were normalized to loaded amount of individual gp120 glycoforms (V5-tag-positive bands). Mean values from two Western blots were calculated and expressed as relative reactivity of individual monoclonal antibodies with each gp120 glycoform.

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AIDS Research and Therapy

ISSN: 1742-6405

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