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Table 2 Proviral DNA tropism assay performance characteristics

From: A genotypic HIV-1 proviral DNA coreceptor tropism assay: characterization in viremic subjects

 

Within assay precision1

Between assay precision2

LOD955

Specimen type

Whole blood

PBMC

Whole blood

PBMC

Whole blood

PBMC

Tropism concordance3

100%

100%

100%

100%

N/A

N/A

DNA sequence concordance4

98.9%

98.9%

99.0%

98.6%

N/A

N/A

DNA copies/mL

N/A

N/A

N/A

N/A

480

360

  1. 1Three replicates of 3 clinical samples were tested in a single run. Two samples were classified as R5 and one was classified as X4.
  2. 2Three replicates of 3 clinical samples were tested on 3 different days in 3 runs. Two samples were classified as R5 and one was classified as X4.
  3. 3The tropism classifications for all replicates were 100% concordant with the original R5 or X4 classification.
  4. 4Nucleotide sequences for the replicates were compared to the consensus sequence and differences were tabulated. Mixed base calls (K, M, R, S, W, Y) accounted for the differences.
  5. 5The sensitivity standards were prepared by diluting purified, quantitated 963 bp HIV-1 proviral DNA amplicons in HIV-1 negative human whole blood or plasma and the LOD95 values were determined by probit analysis.
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AIDS Research and Therapy

ISSN: 1742-6405

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