A genotypic HIV-1 proviral DNA coreceptor tropism assay: characterization in viremic subjects

Table 1 Tropism prediction concordance between 3 proviral DNA (pvDNA) sample types and plasma RNA in paired samples from 41 HIV-1 positive patients

pvDNA source	X4, No. (%)		Concordance	Sensitivity^a	Specificity^b	Kappa^c
pvDNA source	Test	Ref^d	Concordance	Sensitivity^a	Specificity^b	Kappa^c
Whole blood (Tube 1a)	17 (41)	14 (34)	93%	100%	89%	0.85
PBMCs (Tube 2b)	16 (39)	14 (34)	95%	100%	93%	0.90
PBMCs + Plasma (Tube 3)	15 (37)	14 (34)	98%	100%	96%	0.95

^aSensitivity is defined as concordant X4 results between the test and reference methods/reference method X4s.
^bSpecificity is defined as concordant R5 results between the test and reference methods/reference method R5s.
^cKappa: a measure of inter-rater agreement: 0.41-0.60: moderate agreement; 0.61-0.80: good agreement; 0.81 – 1.0 excellent agreement.
^dPlasma RNA testing was used as the reference method for all proviral DNA tests.

ISSN: 1742-6405