Figure 5

Amyloidogenic inhibitor D3 reduces peptide boosted infectivity. (A and B) TZM-bl reporter cells were infected with equal amounts of the dual-tropic HIV-1 lab strain NL4-3 PI 952 [11] in the presence of 10 μg/ml SEVI (A) or Aβ(1–42) fibrils (B). As indicated, the fibrils were preincubated with D3 (0, 1, 10 or 100 μg/ml) prior to infection. Luciferase activity of infected cells was assayed 48 h post infection. (C and D) X-fold change Luciferase RLUs from (A and B). (E) X-fold change Luciferase RLUs of infected TZM-bl cells, which were infected with viruses that were treated with D3 in the absence of fibrils at the indicated concentrations. (F) X-Gal staining of TZM-bl reporter cells that were infected as described above (A and B). (*** p < 0.001, ** p < 0.01 referred to fibril treated and infected cells).