Figure 2

Inflammasome genes expression in IL4-DC and IFN-DC from HIV + subjects pulsed with HIV-1. mRNA expression 4 hours after stimulation of IL4-DC and IFN-DC with chemically inactivated HIV-1 was reported for NLRP1, NLRP3, NLRC4, AIM2, CASP1 and IL1B genes (2A) . Fold-change (FC) in mRNA expression of each gene was calculated with respect to the untreated condition as 2^-∆∆Ct where ∆∆Ct = ∆Ct HIV- ∆Ct Untreated. Results are expressed as average (AVG) of 2^-∆∆Ct ± standard error, n = 20. All the Ct values were normalized against ACTB Ct (∆Ct). Stacked lines showed FC threshold (0.5-1.5). 1-way Anova analysis was used to compare genes expression in IFN-DC and IL4-DC. * = p < 0.05. (2B) Supernatants of IFN-DC and IL4-DC stimulated or not with HIV-1 were analysed for the presence of secreted IL-1β. Results are expressed as the average concentration (pg/ml) ± standard error. 1-way Anova analysis was used to compare IFN-DC and IL4-DC.