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Figure 3 | AIDS Research and Therapy

Figure 3

From: Lentiviral transduction of Tar Decoy and CCR5 ribozyme into CD34+ progenitor cells and derivation of HIV-1 resistant T cells and macrophages

Figure 3

FACS analysis of transgenic macrophages for the CD14 surface marker and EGFP expression: Control and HIV-1 Tar & Tar-CCR5Rz vector transduced CD34+ cells were differentiated into macrophages in vitro in cytokine medium. Cells were stained for the macrophage surface marker CD14 using CD14-PE antibodies. Cells were analyzed by FACS for CD14 and EGFP. Panel A: CD14 staining of macrophages. 1, Nontransduced cells; 2, Control EGFP vector transduced; 3, HIV-1-Tar vector transduced; 4, HIV-1-Tar-CCR5Rz transduced. Percent CD14 positive cells are indicated together with isotype staining controls (unshaded areas). Panel B: EGFP expression by transduced macrophages. 1, Nontransduced cells; 2, Control EGFP vector transduced; 3, HIV-1-Tar vector transduced; 4, HIV-1-Tar-CCR5Rz vector transduced. Percent EGFP positive macrophages are indicated.

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